Bayesian estimation of diagnostic sensitivity and specificity of a qPCR and a bacteriological culture method for Piscirickettsia salmonis in farmed Atlantic salmon (Salmo salar L.) in Chile
Emilie Laurin, Ian Gardner, Andrea Peña, Marco Rozas-Serri, Jorge Gayosa, Joaquin Neumann, Fernando Mardones Abstract Early detection of piscirickettsiosis is an important purpose of government- and industry-based surveillance for the disease in Atlantic salmon farms in Chile. Real-time qPCRs are currently used for surveillance because bacterial isolation is inadequately sensitive or rapid enough for routine use. […]
Atlantic Salmon Pre-smolt Survivors of Renibacterium salmoninarum Infection Show Inhibited Cell-Mediated Adaptive Immune Response and a Higher Risk of Death During the Late Stage of Infection at Lower Water Temperatures
Atlantic Salmon Pre-smolt Survivors of Renibacterium salmoninarum Infection Show Inhibited Cell-Mediated Adaptive Immune Response and a Higher Risk of Death During the Late Stage of Infection at Lower Water Temperatures Marco Rozas-Serri, Carlos Lobos, Rodolfo Correa, Ricardo Ildefonso, Jorge Vásquez, Ariel Muñoz, Lucerina Maldonado, Victoria Jaramillo, Darling Coñuecar, Camila Oyarzún, Romina Walker, Carolina Navarrete, Jorge Gayosa, Patricio Mancilla, […]
Gene expression associated with immune response in Atlantic salmon headkidney vaccinated with inactivated whole-cell bacterin of Piscirickettsia salmonis and pathogenic isolates
Marco Rozas-Serri, Andrea Peña, Lucerina Maldonado Abstract Piscirickettsiosis is the most challenging disease present in the Chilean salmon industry. The aim of this study was to describe the expression of genes associated with immune response of Atlantic salmon intraperitoneally infected with LF-89 and EM-90 Piscirickettsia salmonis and vaccinated with inactivated whole-cell bacterin of P. salmonis. […]
Gill diseases in marine salmon aquaculture with an emphasis on amoebic gill disease
Marco Rozas-Serri Abstract Gill diseases are a growing health challenge in salmon farming worldwide, but many gaps remain in our knowledge. Gill diseases are generally complex and multifactorial disorders, often with presumable spatial and temporal distribution patterns, but are highly difficult to effectively prevent and control. The term complex gill disease (CGD) includes a wide […]
Transcriptomic profiles of post-smolt Atlantic salmon challenged with Piscirickettsia salmonis reveal a strategy to evade the adaptive immune response and modify cell-autonomous immunity
Marco Rozas-Serri, Andrea Peña, Lucerina Maldonado Abstract Piscirickettsiosis is the main bacterial disease affecting the Chilean salmon farming industry and is responsible for high economic losses. The development of effective strategies to control piscirickettsiosis has been limited in part by insufficient knowledge of the host response. The aim of this study was to use RNA […]
Comparison of gene expression in post-smolt Atlantic salmon challenged by LF-89-like and EM-90-like Piscirickettsia salmonis isolates reveals differences in the immune response associated with pathogenicity
Marco Rozas-Serri, Andrea Peña, Gabriel Arriagada, Ricardo Enríquez, Lucerina Maldonado Abstract Piscirickettsiosis is the main bacterial disease affecting the Chilean salmon farming industry and is responsible for high economic losses. The aim of this study was to describe and comparatively quantify the immune response of post-smolt Atlantic salmon infected by cohabitation with fish bearing LF-89-like […]
Comparative pathogenesis of piscirickettsiosis in Atlantic salmon (Salmo salar L.) post-smolt experimentally challenged with LF-89-like and EM-90-like Piscirickettsia salmonis isolates
Marco Rozas-Serri, Ricardo Ildefonso, Andrea Peña, Ricardo Enríquez, Soraya Barrientos, Lucerina Maldonado Abstract Piscirickettsiosis (SRS) is the most prevalent bacterial disease in Chilean salmon aquaculture and is responsible for high economic losses. The aim of this study was to comparatively characterize the pathogenesis of SRS in post-smolt Atlantic salmon during the early and late stages […]
Piscirickettsiosis and Piscirickettsia salmonis in fish: a review
Marco Rozas-Serri, Ricardo Enríquez Abstract The bacterium Piscirickettsia salmonis is the aetio- logical agent of piscirickettsiosis a severe disease that has caused major economic losses in the aquaculture industry since its appearance in 1989. Recent reports of P. salmonis or P. salmonis-like organisms in new fish hosts and geographical regions have increased interest in the bacterium. […]
Epidemiology of Amoebic Gill Disease (AGD) in Chilean salmon industry between 2007 and 2010
Marco Rozas-Serri, Harry Bohle, Horst Grothusen, Patricio Bustos Abstract The recent report of amoebic gill disease (AGD) and of Neoparamoeba perurans in Chile has made it necessary to develop practical tools that will be useful for carrying out epidemiological studies. The prevalence of AGD in Atlantic salmon was 55.7% (29/52 farms) and the epidemic curve was […]
Development of PCR assay for detection of Neoparamoeba perurans and comparison of histological diagnosis
Marco Rozas-Serri, Harry Bohle, Ricardo Ildefonso, Patricio Bustos Abstract The recent description of Amoebic Gill Disease (AGD) and Neoparamoeba perurans in Atlantic salmon (Salmo salar) farmed in Chile has necessitated the development of more reliable and sensitive diagnostic tests. Final diagnosis of infection is normally confirmed by histology. However, the correlation between gross gill lesions […]